High Performance Liquid Chromatography (HPLC)

High Performance Liquid Chromatography is basically a highly improved form of column chromatography.  Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres.  That makes it much faster.
It also allows you to use a very much smaller particle size for the column packing material which gives a much greater surface area for interactions between the stationary phase and the molecules flowing past it. This allows a much better separation of the components of the mixture.
The other major improvement over column chromatography concerns the detection methods which can be used.  These methods are highly automated and extremely sensitive.


  • Designed to provide Highest Speed, Resolution and Sensitivity
  • A new power range allows you to deploy any particle type, any column dimensions, or any mobile and stationary phases
  • Intelligent System Emulation Technology (ISET) provides seamless transfer of methods between LCs, regardless of the brand, delivering unchanged retention time and peak resolution Product brochure
  • Infinite power range that combines ultra high pressure up to 1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance, compatibility, flexibility and investment protection
  • Dynamic range greater than 10,000 for enantiomeric excess and quantitation of impurities
  • Different Solvent Selection choices for a wide range of organic modifiers to exploit unique selectivity
  • Combined Aurora and solvent delivery system for delivery of CO2 flow for highest sensitivity
  • Ten- to fifteen fold lower operating costs by using standard grade gaseous CO2 instead of highly expensive SFC grade dip tube CO2
  • Optional Column Screening Kit or full method development features available for fast, straight forward column and modifier selection
  • MS Compatibility with the 6100 Series Single Quadruple LC/MS systems Low Solvent Consumption and less waste generation for true green chemistry
  • Lab Advisor Software – with intuitive diagnostic and monitor capabilities and alert functions to notify you of problems

About PNF

Pooya Negar Fanavar Company “PNF” established in Tehran on the subject of Purchase, Sales and After Sales Services of Analytical Instruments, Ambient Air Pollution Monitoring Systems, Water Monitoring Systems, Environmental Testing Equipment and Import of related products... more